Confocal Assistant can be downloaded as an installable file by clicking on
GET CONFOCAL ASSISTANT.
Krypton/Argon 15mW 488 568 647 Krypton/Argon 60mW 488 568 647 Argon 25mW 488 514 Argon 100mW 457/488 514 Argon 250mW(UV-VIS) 351/363 488 514 He Cadmium 10mW 325 422 He Neon Green 1mW 543 He Neon Red 10mW 633Note: Information was taken from the BioRad Confocal information booklet. The UV line for the HeCad laser is not noted in the BioRad booklet. It is from Cell Biological Applications of Confocal Microscopy, Edited by Brian Matsumoto, p. 122, Vol 38 Methods in Cell Biology, 1993, Academic Press (ISBN 0-12-480430-6).
FLUOROCHROME EXCITATION EMISSION Diamino Phenyl Oxydiazole (DAO) 280 460 Stilbene 335 440 Dans (1-Dimethyl Amino Naphaline 5 Sulphonic Acid) 340 525 Dansyl NH-CH3 in water 340 578 Dopamine 340 490-520 Noradrenaline 340 490-520 Hoechst 33258 (bound to DNA) 346 460 Aminocoumarin 350 445 DAPI 350 470 Indo-1 350 405-482 Thioflavin TCN 350 460 Granular Blue 355 425 Bisbenzamide 360 600-610 Intrawhite Cf Liquid 360 430 Phorwite AR Solution 360 430 MPS (Methyl Green Pyronine Stilbene) 364 395 BAO 9 (Bisaminophenyloxadiazole) 365 395 Calcophor White Standard Solution 365 435 Nuclear Yellow 365 495 Pyrozal Brilliant Flavin 7GF 365 495 Serotonin 365 520-540 SITS (Stilbene Isothiosulphonic acid) 365 460 True Blue 365 420-430 Uvitex SFC 365 435 Blancophor SV 370 435 Calcien Blue 370 435 Calcofluor RW Solution 370 440 Leucophor PAF 370 430 Phorwite BKL 370 430 Phorwite RPA 375 430 Calcophor White ABT Solution 380 475 Leucophor SF 380 465 Phorwite Rev 380 430 Coumarin-Phalloidin 387 470 Blancophor FFG Solution 390 470 Tetracycline 390 560 Tinopol CBS 390 4.3 Leucophor WS 395 465 5-Hydroxy Tryptamine (5-HT) 400 530 Cascade Blue 400 425 3-Hydroxypyrene 5,8,10-Tri Sulfonic acid 403 513 FIF (Formaldehyde Induced Fluorescence) 405 435 Gloxalic Acid 405 460 Catecholamine 410 470 Primuline 410 550 Pyronine 410 540 Uranine B 420 520 Quinacrine Mustard 423 503 Lucifer Yellow CH 425 528 ACMA 430 474 Berberine Sulphate 430 550 Brilliant Sulphoflavin FF 430 520 Diphenyl Brilliant Flavine 7GFF 430 520 Euchrysin 430 540 Genacryl Brilliant Yellow 10GF 430 485 Genacryl Yellow 5GF 430 475 Lucifer Yellow VS 430 535 Sevron Yellow L 430 490 Thioflavin S 430 550 Thioflavin 5 430 550 Acriflavin 436 520 Atabrine 436 490 Calcofluor White 440 500-520 Sevron Orange 440 530 Astrazon Yellow 7 GLL 450 480 Aurophosphine G 450 580 Maxilon Brilliant Flavin 10 GFF 450 495 Mithramycin 450 570 NBD Amine 450 530 Thiozol Orange 453 480 Auramine 460 550 Coriphosphine O 460 575 Maxilon Brilliant Flavin 8 GFF 460 495 Nylosan Brilliant Flavin E8G 460 510 BOBO 1 462 481 BOPRO 1 462 481 Phosphine 3R 465 565 Acridine Yellow 470 550 Astrazon Orange R 470 540 Genacryl Pink 3G 470 583 Procion Yellow 470 600 Rhodamine 5 GLD 470 565 Sulpho Rhodamine G Extra 470 570 Fluo 3 485 503 Fluorescein Isothiocyanate (FITC) 490 525 YO PRO 1 491 509 Astrazon Brilliant Red 4G 500 585 Sevron Brilliant Red 4G 500 583 Acridine Orange (bound to DNA) 502 526 Bodipy Fl 503 512 BCECF 505 530 Calcium Green 505 532 Ethidium Bromide 510 595 Thiazine Red R 510 580 Rhodamine 123 511 534 TOTO 1 514 533 Astrazon Red 6B 520 595 Genacryl Brilliant Red B 520 590 Sevron Brilliant Red 2B 520 595 sulphO Rhodamine B Can C 520 595 Magdala Red 524 600 Eosin 525 545 Rhodamine 6G 526 555 Erythrosin ITC 530 558 Rhodamine WT 530 555 Sevron Brilliant Red B 530 590 Propidium Iodide 536 617 Acid Fuchsin 540 630 Rhodamine B 540 625 Rhodamine BB 540 580 Rhodamine BG 540 572 Rose Bengal 540 550-600 Xylene Orange 546 580 Rhodamine B Extra 550 605 CY3.1 8 554 568 Aminoactinomycin D 555 655 TRITC (Tetramethyl Rhodamine Isothiocyanate) 557 576 Snarf 1 563 639 Pararosaniline (Feulgen) 570 625 Lissamine Rhodamine B200 (RD200) 575 595 XRITC 582 601 Texas Red 596 615 TOTO 3 642 661 CY5.1 8 649 666 Allophycocyanin 650 661 Ultralite 656 678 CY7 710 805 Nuclear Fast Red 289-530 580 Dimethylamino-5-Sulphonic acid 310-370 520 Dansa (Diamino Naphtyl Sulphonic Acid) 340-380 430 Fura-2 340-380 512 Flazo Orange 375-530 612 Chinacrine 450-490 515 Nitrobenzoxadidole 460-470 510-650 Phycoerythrin R 480-565 578 Nile Red 515-530 525-605 Rhodamine B 200 523-557 595 Haematoporphyrin 530-560 580 Pontochrome Blue Black 535-553 605 Pyronine B 540-590 560-650 AFA (Acriflavin Feulgen SITSA) 355-425 460 Anthroyl Stearate 361-381 446 Aurophosphine 450-490 515 Acridine Red 455-600 560-680 Alizarin Complexon 530-560 580 Alizarin Red 530-560 580 Thiolyte 370-385 477-484 5-Hydroxy Tryptamine 380-415 520-530 SITS (Primuline) 395-425 450Listed Alphabetically
FLUOROCHROME EXCITATION EMISSION 3-Hydroxypyrene 5,8,10-Tri Sulfonic acid 403 513 5-Hydroxy Tryptamine 380-415 520-530 5-Hydroxy Tryptamine (5-HT) 400 530 Acid Fuchsin 540 630 Acridine Orange (bound to DNA) 502 526 Acridine Red 455-600 560-680 Acridine Yellow 470 550 Acriflavin 436 520 AFA (Acriflavin Feulgen SITSA) 355-425 460 Alizarin Complexon 530-560 580 Alizarin Red 530-560 580 Allophycocyanin 650 661 ACMA 430 474 Aminoactinomycin D 555 655 Aminocoumarin 350 445 Anthroyl Stearate 361-381 446 Astrazon Brilliant Red 4G 500 585 Astrazon Orange R 470 540 Astrazon Red 6B 520 595 Astrazon Yellow 7 GLL 450 480 Atabrine 436 490 Auramine 460 550 Aurophosphine 450-490 515 Aurophosphine G 450 580 BAO 9 (Bisaminophenyloxadiazole) 365 395 BCECF 505 530 Berberine Sulphate 430 550 Bisbenzamide 360 600-610 BOBO 1 462 481 Blancophor FFG Solution 390 470 Blancophor SV 370 435 Bodipy Fl 503 512 BOPRO 1 462 481 Brilliant Sulphoflavin FF 430 520 Calcien Blue 370 435 Calcium Green 505 532 Calcofluor RW Solution 370 440 Calcofluor White 440 500-520 Calcophor White ABT Solution 380 475 Calcophor White Standard Solution 365 435 Cascade Blue 400 425 Catecholamine 410 470 Chinacrine 450-490 515 Coriphosphine O 460 575 Coumarin-Phalloidin 387 470 CY3.1 8 554 568 CY5.1 8 649 666 CY7 710 805 Dans (1-Dimethyl Amino Naphaline 5 Sulphonic Acid) 340 525 Dansa (Diamino Naphtyl Sulphonic Acid) 340-380 430 Dansyl NH-CH3 in water 340 578 DAPI 350 470 Diamino Phenyl Oxydiazole (DAO) 280 460 Dimethylamino-5-Sulphonic acid 310-370 520 Diphenyl Brilliant Flavine 7GFF 430 520 Dopamine 340 490-520 Eosin 525 545 Erythrosin ITC 530 558 Ethidium Bromide 510 595 Euchrysin 430 540 FIF (Formaldehyde Induced Fluorescence) 405 435 Flazo Orange 375-530 612 Fluorescein Isothiocyanate (FITC) 490 525 Fluo 3 485 503 Fura-2 340-380 512 Genacryl Brilliant Red B 520 590 Genacryl Brilliant Yellow 10GF 430 485 Genacryl Pink 3G 470 583 Genacryl Yellow 5GF 430 475 Gloxalic Acid 405 460 Granular Blue 355 425 Haematoporphyrin 530-560 580 Hoechst 33258 (bound to DNA) 346 460 Indo-1 350 405-482 Intrawhite Cf Liquid 360 430 Leucophor PAF 370 430 Leucophor SF 380 465 Leucophor WS 395 465 Lissamine Rhodamine B200 (RD200) 575 595 Lucifer Yellow CH 425 528 Lucifer Yellow VS 430 535 Magdala Red 524 600 Maxilon Brilliant Flavin 10 GFF 450 495 Maxilon Brilliant Flavin 8 GFF 460 495 MPS (Methyl Green Pyronine Stilbene) 364 395 Mithramycin 450 570 NBD Amine 450 530 Nile Red 515-530 525-605 Nitrobenzoxadidole 460-470 510-650 Noradrenaline 340 490-520 Nuclear Fast Red 289-530 580 Nuclear Yellow 365 495 Nylosan Brilliant Flavin E8G 460 510 Pararosaniline (Feulgen) 570 625 Phorwite AR Solution 360 430 Phorwite BKL 370 430 Phorwite Rev 380 430 Phorwite RPA 375 430 Phosphine 3R 465 565 Phycoerythrin R 480-565 578 Pontochrome Blue Black 535-553 605 Primuline 410 550 Procion Yellow 470 600 Propidium Iodide 536 617 Pyronine 410 540 Pyronine B 540-590 560-650 Pyrozal Brilliant Flavin 7GF 365 495 Quinacrine Mustard 423 503 Rhodamine 123 511 534 Rhodamine 5 GLD 470 565 Rhodamine 6G 526 555 Rhodamine B 540 625 Rhodamine B 200 523-557 595 Rhodamine B Extra 550 605 Rhodamine BB 540 580 Rhodamine BG 540 572 Rhodamine WT 530 555 Rose Bengal 540 550-600 Serotonin 365 520-540 Sevron Brilliant Red 2B 520 595 Sevron Brilliant Red 4G 500 583 Sevron Brilliant Red B 530 590 Sevron Orange 440 530 Sevron Yellow L 430 490 SITS (Primuline) 395-425 450 SITS (Stilbene Isothiosulphonic acid) 365 460 Stilbene 335 440 Snarf 1 563 639 sulphO Rhodamine B Can C 520 595 Sulpho Rhodamine G Extra 470 570 Tetracycline 390 560 TRITC (Tetramethyl Rhodamine Isothiocyanate) 557 576 Texas Red 596 615 Thiazine Red R 510 580 Thioflavin S 430 550 Thioflavin TCN 350 460 Thioflavin 5 430 550 Thiolyte 370-385 477-484 Thiozol Orange 453 480 Tinopol CBS 390 4.3 TOTO 1 514 533 TOTO 3 642 661 True Blue 365 420-430 Ultralite 656 678 Uranine B 420 520 Uvitex SFC 365 435 Xylene Orange 546 580 XRITC 582 601 YO PRO 1 491 509Back to Biology Home Page