Misc. Ray Troll,
Histology Links;
Int. Soc. Neuroethology;
Scott Camazine;
FedEx Tracking;
University Home Pages; PIR;
PIR ESC(Vogt-smadhuri);
home videos;
USC New Media;
Real Admin;
Bandwidth Port Distribution;
Bandwidth by Router;
Spain Videos;
RAG.
Memberships: AChemS, Society for Neuroscience
|
|
|
Like OBPs, ODEs are also antennal specific, and are able to degrade odor molecules extremely fast (see Vogt (SE); and Rybczynski (AOX)). I identified a male antennal specific Esterase in the silkmoth Antheraea polyphemus and my friend and colleague Bob Rybczynski identified an antennal specific Aldehyde Oxidase (AOX) in the moth Manduca sexta. In a joint effort, Bob and I suggested that one can predict what functional type enzyme is present if structures of the sex-pheromone for that species are known ( see Rybczynski & Vogt). Recently, Matt Rogers, as a graduate student in my lab, identified an antennal specific GST in M. sexta, restricted to the pheromone sensitive sensilla, and suggested that this enzyme might have a dual function of inactivating harmful xenobiotics entering the antenna, as well as contributing to the degradation of the aldehyde pheromone molecules of this species ( see Rogers & Vogt). [Matt is currently a postdoc at Columbia University with Stuart Firestein studying various aspects of olfactory transduction systems.]
SNMP. Matt Rogers and I also identified and characterized an antennal specific protein that is abundantly expressed in the receptor membranes of olfactory neurons of moths (see Rogers, Sun, Lerner & Vogt, 1997 and Rogers, Steinbrecht & Vogt, 2001 and Rogers, Krieger & Vogt, 2001); we called this protein SNMP. This work initiated as follow up of our previous specific labeling of a sensory membrane protein using a photoaffinity analog of the sex pheromone ( see Vogt, Riddiford & Prestwich) SNMP is related to the CD36 class of receptor proteins, may of which interact with other proteins. We are exploring the function of SNMP, which we believe may interact with other proteins of the olfactory sensilla, perhaps the OBPs assisting the off-loading of odor molecules near the ORs.
These studies are revealing molecular landscapes that have evolved to support olfactory based behaviors in an extremely large and diverse group of animals. The situation of these molecules at the literal interface between animal and environment makes these molecules particularly useful in understanding the molecular-genetic evolution of olfactory based behaviors.
Matt Rogers is currently a postdoc in the lab of Stuart Firestein at Columbia University
Frank Callahan, Dick Dickens and I recently cloned
an OBP from the
Lygus bug. This was significant because up until then, OBPs were only known from several insect orders belong to the holometabolous group of insects known as Endopterygota. Insects are evolutionarily divided into non-winged (more primitive) and winged (less primitive). The winged insects are further divided into the Paleoptera (more primitive) and the Neoptera (less primitive). Neopterous insects comprise >98% of all known insect species. The Endopterygota represent one group of neopterous insects (>83% of all insect species) and includes moths, beetles, bees, ants and flies, but excludes cockroaches, grasshoppers, crickets, earwigs and termites. The bugs (hemipterans) are considered a sister group with the Endopteryota, and with the Endopterygota, include >94% of all insect species. Previous to our effort, OBPs were only known from 4 insect orders, including moths, beetles, flies and bees. As a consequence of our effort, demonstrating an OBP from a bug (hemipteran), we can say that the OBP gene was present in the ancestor of both the Endopterygota and Hemipteran insects and that these genes are therefore represented in >94% of all insects, which is a really huge number of species.
Marie-dominique Franco (as postdoc in my laboratory) and I showed that mitotic activity which gives rise to the cells comprising individual sensilla occurs as a temporal-spatial wave in M. sexta, initiating along the annular and progressing to fill each annulus. This mitotic activity initiates at a low level around 24 hrs after pupation (a.p.), but increases dramatically around 48 hr a.p. and is complete around 72-80 hrs a.p., coincident with antennal apolysis (the detachment of the antennal epidermis from the overlying pupal cuticle. The activity is sensitive to rising ecdysteroids, and does not occur in animals entering diapause (but activates with the onset of development following diapause. My students (especially Jonathan Bohbot and Kenny Fernandez) and I have continued these studies looking at the temporal-spatial patterns of expression of key transcription factors (Distal-less, Bric-a-brac, Extradenticle, Broad, EcR), as well as the development of the antennal imaginal disc during the final larval instar. We are also comparing this development (lepidopteran) with that which occurs in the fruit fly Drosophila melanogaster (dipteran). There are many aspects of antennal development that appear quite different between Manduca and Drosophila, and we would like to understand the nature of these differences.
[Marie-dominique Franco continued her studies on olfactory development for several years with Dr. Gail Burd at University of Arizona, studying olfactory metamorphosis in the frog. She spent one year as a visiting professor teaching biology at Grinell College, and has been an Assistant Professor at Regis University in Denver in Biology since 2001 (CLICK HERE). Jonathan Bohbot completed his Ph.D. in 2004 and is currently a postdoc with Larry Zwiebel at Vanderbilt University, working on mosquito odor receptors. Kenny Fernandez is continuing his Ph.D. studies.]
There are many odor receptor genes in a given species, at least 1000 in rat and around 100 in zebrafish. And, we know that at least most olfactory receptor neurons only express one of these odor receptor genes, and depending on which gene the neuron expresses, the neuron's axon projects to a specific site in the brain (a specific glomerulus in the olfactory bulb). A question that remains unanswered is how do olfactory neurons choose which of many odor receptors they should express. Chris, Sara Lindsay and I addressed this question in part by asking whether neurons expressing specific odor receptors at the onset (early in development) were situated in spatially conserved locations in the olfactory epithelium. We determined that there is no spatial conservation in the positions of these neurons, leaving the question of how choice is made unanswered, but affirming that this choice must be made by a mechanism other than the spatial position of the neuron.
The zebrafish remains a great system to explore olfactory development and I remain very open to working with this system, although most of my/our work is currently directed at the insect projects described above. Nevertheless, Sara Lindsay and I recently published a study showing that newly hatched zebrafish can display behavioral responses to chemosensory stimulants only 5 days after fertilization. We are also helping Lee Furguson establish a zebrafish colony suitable for studying the effects of low level estrogens and other environmental compounds on protein expression and early development.
[Chris Byrd is currently an Associate Professor of Biological Sciences at Western Michigan University.
Sara Lindsay is currently an Assistant Professor of Marine Biology at the University of Maine.]
We are characterizes the Odor Receptor gene family in 3 of the 6 species of Sea Turtles: Loggerhead, Leatherback and Green. We are asking a variety of questions about each, to estimate the importance olfaction is to each species.
1. What is the size of the OR gene family in turtles? The number of OR genes in fish, about 100, has expanded to about 1000 genes in mammals. OR genes are known from fish, coelacanth, amphibia, chicken and several mammals. Turtles belong to a ancestral to chickens and mammals, but more recent than amphibia, coelacanth and fish. The number of genes may be informative to the evolutionary position turtles hold in this scheme.
2. Do sea turtle ORs detect water-borne or air-borne odorants? Two classes of OR genes have been identified relative to whether they interact with air-borne or water-borne odorants; these classes can be distinguished by sequence. Receptors for water-borne odorants are present in fish, coelacanth and amphibian. Receptors for air-borne odorants are present in all tetrapod lineages from coelacanth on. The air-borne ORs of coelacanth are all inactive pseudogenes. Most if not all the ORs (air-borne) of dolphins are inactive pseudogenes (dolphins olfactory bulb degenerates embryonically). What is the situation in sea turtles? What classes are present and which are expressed?
3. What is the allelic diversity of specific OR genes? The relative importance of olfaction might be estimated by the degree of allelic diversity for the OR genes. A high level of diversity might indicate a low level of importance, and vice versa.
This work is part of a project, headed by Richard Brill of the National Marine Fisheries Service (Honolulu) to assess sensory systems in sea turtles to the end of identifying more effective methods for keeping endangered sea turtles out of fishing nets.
Graduate student Michelle Vieyra has been working on this project now for 3 years. She was initially assisted by Dan Anderson (now in Medical School) and subsequenlty by graduate student Paul Kobres (currently a Ph.D. student in the lab studying nucleases in caterpillar saliva). During the initial summer of the project (2001), undergraduate Trevan Lyn joined the project as an NSF supported student. Trevan, a Jamacan student attending Benedict College in Columbia SC, participated in our NSF funded "Undergraduate Mentoring in Environmental Biology" program, working in my lab on a parallel project cloning ORs from alligators, providing a comparison between two reptilian lineages.
You can download a concept paper on this project HERE.
Neurobiology was developed by me in 1992, is restricted to 12 students, and includes formal lectures mixed with student presentations and an extensive laboratory that encourages student originated exploration. The course emphasizes cellular and synaptic processes and interactions that form the basis for simple behaviors, and might be viewed as an advanced introductory course in molecular neuroethology. The course has always had an inquiry basis, a feature significantly augmented in 2004 by the addition of PDFs of a multitude of major literature starting the 1936 manuscript of J.Z. Young on the anatomy of the squid, and the 1912 manuscript of Adrian and Lucus on summation in muscle contractions.
Comparative Physiology was developed by me in 2000. This course is limited to about 45 students and emphasizes basic principals that are common to the physiology of diverse animals (the Kroghian rather than the Schmidt-Nielson kind of Comp. Phys.). The unique aspect of this course is that the lectures are delivered on line via streamed video. Students view these lectures outside scheduled class time, freeing class time for interactive problem solving. In a typical class, I arbitrarily hand out numbers from 1 to 8 to form student groups (different make up for each class); each of these student groups is then given a question to work out on the black board (my room has huge blackboards). A wonderful chaos ensues as student groups simultaneously work there answers and I go around clarifying the questions. I feel I am training students to think in unfamiliar ways, while teaching a form of iconography (view some of the lectures for your self to understand this last comment). The course tends to emphasize molecular processes, often in the context of hormonal regulation. The course is based on the premise that most of what one needs to know about physiology can fit on one 3"x5" note card (perhaps both sides), provided one also has an active and thinking brain (i.e., understanding the rules empowers you to hypothesize the facts).
For more, click below...
|
|
|
Nichols Z, Vogt RG (2008) The SNMP / CD36 gene family in Diptera, Hymenoptera and Coleoptera: Drosophila melanogaster, D. pseudoobscura, Anopheles gambiae, Aedes aegypti, Apis mellifera, and Tribolium castaneum. Insect Biochemistry and Molecular Biology 38, 398-415.
Link to article.
Franco M-d, Bohbot J, Fernandez K, Hanna J, Poppy J, Vogt R (2007) Sensory Cell Proliferation within the Olfactory Epithelium of Developing Adult Manduca sexta (Lepidoptera). PLoS ONE 2(2): e215. doi:10.1371/journal.pone.0000215.
PDF
Vogt R (2006) How sensitive a nose? Science STKE pe8.
Read Abstract
Vierya M, Vogt R (2006) The importance of odor receptors to the chemosensory behavior of sea turtles. In Sea Turtle and Pelagic Fish Sensory Biology: Developing Techniques to Reduce Sea Turtle Bycatch in Longline Fisheries. (ed. Yonat Swimmer, Richard Brill). U.S. Dep. Commer., NOAA Technical Memorandum NOAA-TM-NMFS-PIFSC-7, 27-40.
PDF of Manuscript
PDF of Full Memorandum
Bohbot J, Vogt R (2005) Antennal expressed genes of the Yellow Fever Mosquito (Aedes aegypti L.); characterization of Odorant-Binding Protein 10 and Takeout. Insect Molecular Biology and Biochemistry, 35, 961-979.
Read Abstract
Merritt, TJS, Young CR, Vogt RG, Wilkerson RC, Quattro JM (2005) Intron Retention Identifies a Malaria Vector within the Anopheles (Nyssorhynchus) albitaris Complex (Diptera: Culicidae). Molecular Phylogenetics and Evolution, 35, 719-724.
Read Abstract
Vogt RG (2005) Molecular basis of pheromone detection in insects. In Comprehensive Insect Physiology, Biochemistry, Pharmacology and Molecular Biology. Volume 3. Endocrinology. (LI Gilbert, K Iatro, S Gill eds). pp. 753-804. Elsevier, London. ISBN: 044451516X
Lindsay SM and Vogt RG (2004) Behavioral responses of newly hatched zebrafish (Danio rerio) to amino acid chemostimulants. Chem. Senses 29, 93-100.
Read Abstract
Vogt RG (2003) Biochemical Diversity of Odor Detection: OBPs, ODEs and SNMPs. In Insect Pheromone Biochemistry and Molecular Biology. (eds. GJ Blomquist and RG Vogt), pp. 391-446. Elsevier Academic Press, London.
Bloomquist GJ, Vogt RG (2003) Biosynthesis and detection of pheromones and plant volatiles: introduction and overview. In Insect Pheromone Biochemistry and Molecular Biology. In Insect Pheromone Biochemistry and Molecular Biology. (eds. GJ GJ Blomquist and RG Vogt), pp. 3-18. Elsevier Academic Press, London.
BOOK: Insect Pheromone Biochemistry and Molecular Biology. (2003)( eds. GJ Blomquist and RG Vogt), Elsevier Academic Press, London. ISBN 0121071510.
CLICK for Contents, Authors, Jacket
(ISBN 0121071510, $100 @ amazon.com)
Vogt RG (2002) Odorant Binding Proteins of the Malaria Mosquito Anopheles gambiae; Possible Orthologues of the OS-E and OS-F OBPs of Drosophila melanogaster. Journal of Chemical Ecology 28, RC29-RC35.
Download pdf from the JCE web site (go to Rapid Communications) or directly by clicking
"HERE"
Vogt RG, Vieyra M, Anderson D (2002) New discoveries in olfactory capability of sea turtles. Pelagic Fisheries Research Program Newsletter 7(3), 6-9.
PDF of Paper;
(PDRP Newsletter Archive);
(Pelagic Fisheries Research Program)
Vogt RG, Rogers ME, Franco M-d, Sun M (2002). A Comparative Study of Odorant Binding Protein Genes: Differential Expression of the PBP1 - GOBP2 Gene Cluster in Manduca sexta (Lepidoptera) and the organization of OBP genes in Drosophila melanogaster (Diptera). Journal of Experimental Biology 205, 719-744.
Read Abstract
Rogers ME, Krieger J, Vogt RG (2001) Antennal SNMPs (Sensory Neuron Membrane Proteins) of Lepidoptera define a unique family of invertebrate CD36-like proteins. Journal of Neurobiology 49, 47-61.
Read Abstract
Jacquin-Joly E, Vogt RG, Marie-Christine Francois M-C, Nagnan-Le Meillour P (2001) Functional and expression pattern analysis of chemosensory proteins expressed in antennae and pheromonal gland of Mamestra brassicae. Chemical Senses, 26, 833-844.
Read Abstract
Rogers ME, Steinbrecht RA, Vogt RG (2001) Expression of SNMP-1 in olfactory neurons and sensilla of male and female antennae of the silkmoth Antheraea polyphemus. Cell and Tissue Research, 303, 433-446. Read Abstract
Callahan FE, Vogt RG, Tucker ML, Dickens JC, A. K. Mattoo AK. (2000) High level expression of "male specific" pheromone binding proteins (PBPs) in the antennae of female noctuiid moths. Insect Biochemistry and Molecular Biology 30, 507-514. Read Abstract
Vogt RG, Callahan FE, Rogers ME, and Dickens JC. (1999) Odorant Binding Protein Diversity and Distribution among the Insect Orders, as indicated by LAP, an OBP-related protein of the True Bug Lygus lineolaris (Hemiptera, Heteroptera). Chemical Senses 24, 481-495. Read Abstract
Rogers ME, Jani MK. and Vogt RG (1999) An olfactory specific glutathione S-transferase in the Sphinx moth Manduca sexta. Journal of Experimental Biology, 202, 1625-1637. Read Abstract
Franco M-d, Rogers ME, Shimizu C, Shike H, Vogt RG, Burns JC (1998) Infection of Lepidoptera with a Pseudoptyped Retroviral Vector. Insect Biochemistry and Molecular Biology, 28, 819-825. Read Abstract
Merritt TJS, LaForest S, Prestwich GD, Quattro JM, Vogt RG. (1998) Patterns of gene duplication in lepidopteran Pheromone Binding Proteins. Journal of Molecular Evolution. 46, 272-276. Read Abstract
Dickens JC, Callahan FE, Wergin WP, Murphy CA, Vogt RG (1998) Odorant-binding proteins of true bugs. Generic specificity, sexual dimorphism, and association with subsets of chemosensory sensilla. Ann N Y Acad Sci 855, 306-310. Read Abstract
Dickens JC, Callahan FE, Wergin WP, Murphy CA, Vogt RG. (1998) Immunolocalization and Intergeneric Distribution of a Putative Odorant-Binding Protein in True Bugs (Heteroptera: Miridae). Journal of Experimental Biology, 201, 33-41. Read Abstract
Rogers M, Sun M, Lerner MR, Vogt RG. (1997) Snmp-1, a novel membrane protein of olfactory neurons of the silk moth Antheraea polyphemus with homology to the CD36 family of membrane proteins. Journal of Biological Chemistry 272(23), 14792-14804. Read Article
Vogt RG, Lindsay SM, Byrd CA, Sun M. (1997) Spatial patterns of olfactory neurons expressing specific odor receptor genes in 48 hr old embryos of zebrafish Danio rerio. Journal of Experimental Biology 200, 433-443. Read Abstract
Byrd C, Jones J, Quattro J Rogers M, Brunjes P, Vogt R (1996) Ontogeny of odorant receptor gene expression in the zebrafish, Danio rerio. Journal of Neurobiology 29, 445-458. Read Abstract
Miller RM, Vogt RG (1996) Purification and partial sequence of the peptide sperm attractant "Stratrak" from the starfish Puncnopodia helianthoides (Brant, 1896). Journal of Experimental Biology 199, 311-318. Read Abstract
Vogt RG (1995) Molecular genetics of moth olfaction: a model for cellular identity and temporal assembly of the nervous system. in Molecular Model Systems in the Lepidoptera, ed. MR Goldsmith & AS Wilkins. pp. 341-367 Cambridge University Press, Cambridge. (ISBN: 0-521-40249-2)
Vogt RG (1993) Odor receptor proteins recloned: molecular realities of olfactory discrimination in fish. BioEssays 15, 487-489.
Vogt RG, Rybczynski R, Cruz M, Lerner MR. (1993) Ecdysteroid regulation of olfactory protein expression in the developing antenna of the tobacco hawk moth, Manduca sexta. J. Neurobiology, 24, 581-597. Read Abstract
Vogt RG, Rybczynski R, Lerner MR (1991) Molecular cloning and sequencing of general-odorant binding proteins GOBP1 and GOBP2 from the tobacco hawk moth Manduca sexta: Comparisons with other insect OBPs and their signal peptides. Journal of Neuroscience11, 2972-2984. Read Abstract
Vogt RG, Prestwich GD, Lerner MR. (1991) Odorant Binding Protein subfamilies associate with distinct classes of olfactory receptor neurons in insects. Journal of Neurobiology 22, 74-84. Read Abstract
Rybczynski R, Vogt RG, Lerner MR. (1990) Antennal-specific pheromone-degrading aldehyde oxidases from the moths Antheraea polyphemus and Bombyx mori. Journal of Biological Chemistry 265:19712-19715. Read Abstract or Download FULL PDF
Vogt RG, Koehne AC , Dubnau JT & Prestwich GD (1989) Expression of pheromone binding proteins during antennal development in the gypsy moth Lymantria dispar. J. Neurosci. 9, 3332-3346. Read Abstract
Vogt RG, Prestwich GD, LM Riddiford. (1988) Sex pheromone receptor proteins, visualization using a radiolabeled photoaffinity analog. Journal of Biological Chemistry 263, 3952-3959. Read Abstract or Download FULL PDF
Baker TC & Vogt RG (1988) Measured behavioral latency in response to sex-pheronone loss in the large silk moth Antheraea polyphemus (Lepidoptera: Saturniidae). J. Exp. Biol. 137, 29-38. Read Abstract
Vogt RG & Prestwich GD (1988) Variation in olfactory proteins: evolvable elements encoding insect behavior. Olfaction and Taste IX, Annals N.Y. Acad. Sci. 510, 689-691.
Vogt RG & Riddiford LM (1986) Pheromone reception: a kinetic equilibrium. In Mechanisms in Insect Olfaction TL Payne, M Birch & CEJ Kennedy, eds., pp. 201-208. Clarendon Press, Oxford.
Vogt RG & LM Riddiford LM (1986) Scale esterase: a pheromone degrading enzyme from the wing scales of the silk moth Antheraea polyphemus. J. Chem. Ecol. 12, 469-482.
Vogt RG, Riddiford LM, Prestwich GD (1985) Kinetic properties of a sex pheromone-degrading enzyme: the sensillar esterase of Antheraea polyphemus. Proc. Natl. Acad. Sci. USA 82, 8827-8831. Read Abstract ...or... Download FULL PDF
Mills CE & Vogt RG (1984) Evidence that ion regulation in hydromedusae and ctenophores do not facilitate vertical migration. Biol. Bull. 166, 216-227.
Vogt RG, Riddiford LM (1981) Pheromone binding and inactivation by moth antennae. Nature 293, 161-163. Download FULL PDF
