BARBARA CRISCUOLO WALDMAN

Barbara Criscuolo Waldman

Research Associate Professor
Ph.D., 1985, Johns Hopkins University
803-777-9104
bwaldman@biol.sc.edu

Regulation of Glycosylation in Mammalian Cells; Golgi Apparatus; Posttranslational Modifications of Proteins; Protein Trafficking

My research interests center around understanding the biosynthesis, structure and function of membrane proteins and glycoproteins in mammalian cells, and how perturbations of these processes may lead to abnormal cellular physiology. The addition of sugars to an asparagine residue of a polypeptide chain (asparagine-linked glycosylation) is a posttranslational modification which is found on many membrane and secretory proteins made by mammalian cells. We are studying the regulation of the biosynthesis of this class of proteins, particularly within the Golgi apparatus. Nucleotide sugars are the sugar-donor substrates for glycosylation, and their transport into the Golgi apparatus is required for the synthesis of most glycoproteins and glycolipids. Perturbations of this transport have widespread and sometimes detrimental effects. Currently, my lab is determining the molecular mechanisms by which nucleotide sugars are transported across the Golgi membrane and isolating the genes for these transporter proteins. We also study the role of another posttranslational modification of proteins, poly(ADPribosylation), in DNA recombination in mammalian cells, and have recently have become involved in studies which focus on the growth hormone releasing hormone receptor, a plasma membrane protein found on certain anterior pituitary cells. By utilizing a combination of biochemistry, cell biology, genetics and molecular biology this research will lead to an understanding of the various ways in which mammalian cells can regulate the biosynthesis, trafficking, structure and function of membrane proteins and glycoproteins.

Selected Publications:

Lin, Y., Waldman, B.C., and Waldman, A.S. (2002) "Suppression of high-fidelity double-strand break repair in mammalian chromosomes by pifithrin-a, a chemical inhibitor of p53" DNA Repair, in press.

Ross, K.C., Waldman, B.C., Conejero-Goldberg, C., Freed, W., and Coleman, J.R. (2002) "Transplantation of M213-2O Cells with Enhanced GAD67 Expression into the Inferior Collliculus Alters Audiogenic Seizures." Experimental Neurology 177:338-340.

Lukacsovich, T., Waldman, B.C., and Waldman, A.S. (2001) "Efficient Recruitment of Transfected DNA to a Homologous Chromosomal Target in Mammalian Cells." Biochim. Biophys.Acta 1521: 89-96.

Waldman, B.C. (1999) "Isolation of pcDNAI-based Mammalian Expression Vectors from Esherichia coli strain MC1061/P3." BioTechniques 26:402-406.

Waldman, A.S., and Waldman, B.C. (1998) "Stable transfection of mammalian cells by syringe-mediated mechanical loading of DNA". Analytical Biochemistry 258:216-222.

Waldman, B.C., O'Quinn, J.R., and Waldman, A.S. (1996) "Enrichment for gene targeting in mammalian cells by inhibition of poly(ADP-ribosylation)." Biochimica et Biophysica Acta 1308:241-250.

Waldman, A. S., and Waldman, B. C. (1991) "Stimulation of Intrachromosomal Homologous Recombination in Mammalian Cells by an Inhibitor of Poly(ADP-ribosylation)." Nucleic Acids Research 19:5943-5947.

Waldman, B. C., and Waldman, A.S. (1990) "Illegitimate and Homologous Recombination in Cultured Mammalian Cells: Differential Sensitivity to an Inhibitor of Poly(ADP-ribosylation)." Nucleic Acids Research 18:5981-5988.

Waldman, B. C., and Rudnick, G. (1990) "UDP-N-Acetylglucosamine Transport Across the Golgi Membrane: Electroneutral Exchange for Dianionic UMP." Biochemistry 29:44-52.

Waldman, B. C., and Rudnick, G. (1989) "A Method for Replacing Intravesicular Contents of Golgi Vesicles Using an Air-Driven Ultracentrifuge." Analytical Biochemistry 180:216-221.

Waldman, B. C., Oliver, C., and Krag, S. S. (1987) "A Clonal Derivative of Tunicamycin-resistant Chinese Hamster Ovary Cells with Increased N- Acetyl-glucosamine-Phosphate Transferase Activity Has Altered Asparagine-linked Glycosylation." Journal of Cellular Physiology 131:302-317.

Criscuolo [Waldman], B. A., and Krag, S.S. (1982) "Selection of Tunicamycin-resistant Chinese Hamster Ovary Cells with Increased N-Acetylglucosaminyl-transferase Activity." Journal of Cell Biology 94:586-591.

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